The main consequence of the Civil Rights Act of 1964 in the U.S. was the legal prohibition of discrimination based on race, color, religion, sex, or national origin. This landmark legislation was pivotal in dismantling segregation in public spaces and schools, ensuring equal employment opportunities, and establishing the Equal Employment Opportunity Commission (EEOC) to address complaints of discrimination. By providing a legal framework for civil rights advocacy and enforcement, the act signified a major step forward in the struggle for equality and justice for African Americans and other marginalized groups.